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Getting started in cryo-EM, Caltech

4.9
204 ratings
41 reviews

About this Course

This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules....
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41 Reviews

By ANIL KUMAR S

Sep 12, 2018

very good for the beginner like me

By William Navarre

Aug 31, 2018

Loved it. Although it's not heavy on math it helps to have a basic understanding of waves, how they propagate, how they are mathematically represented by sin/cos functions and how interference between two waves works.

By Gerardo Martinez Herrera

Mar 28, 2018

There is a few free courses, this one of the best that I've taken. It would be better if one finishes the course, one could receive any certification

By Lance Richardson

Feb 19, 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

By Nikos Nikolopoulos

Oct 30, 2017

CryoEM made crystal clear! ;P

By stanwang

Oct 19, 2017

excellent

By Vadim Sukhorukov

Jun 23, 2017

Great job! Clear explanations, drawings, schemes.

By Jonathan Foster

May 18, 2017

Great course, very informal - really improved my knowledge in the field of EM

By Robert Fagiewicz

Apr 04, 2017

Great course with clear explanations and very entertaining topics! Didn't expect to cover so many aspects of cryo-EM, would recommend it to everyone interested/working in structural biology or science in general.

By Serhiy Surkov

Jan 26, 2017

I have worked a bit in cryo-em before and I find this course absolutely amazing! Very detailed and easy to understand.