About this Course
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本课程的实验设计贯穿以研究基因功能为目的遗传学思维理念及精髓。通过背景知识的介绍和相关的实验操作,使大家建立这样的理念:研究基因功能时,在分子水平通过敲除或者敲入的策略,使基因突变或者修饰,这些突变依赖载体染色体的传递,将稳定地传给下一代。通过设计精巧的遗传杂交实验,追踪易于识别的染色体标记,就可以定位目的基因、获得纯合突变体,研究表型与基因型间的对应关系,从而理解各种表型的分子机制。整个实验过程涉及生物学不同学科知识和实验设计的融合。 针对遗传学研究的这些关键点,本课程设计了一系列实验,主要以模式动物果蝇为窗口,通过遗传杂交实验,以遗传信息传递的载体染色体为线索,除验证遗传学基本定律外,还引入科研项目的理念和实验环节,以便让大家参与平时在课堂上接触不到的真实科研实践。每次实验包括背景原理、目的介绍、操作要点和相关的视频演示。让学生亲自保管和观察杂交果蝇培养瓶,写生活史观察日记也是本课程的特色之一。 学生,尤其是高年级本科生和进入国内外科研第一线的研究生,均反馈受益非浅。 参考教材为由张文霞主编,高等教育出版社出版的《遗传学实验指导》。此外相应的实验将提供参考文献。 MOOC实验课虽然无法亲自操作,但我们将提供丰富的图片和视频资料,使大家了解其过程。有实验条件的选课人员,可以依照讲解和演示,亲自操作。...
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Beginner Level

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Approx. 14 hours to complete

Suggested: 6 hours/week...
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Chinese (Simplified)

Subtitles: Chinese (Simplified)...
Globe

100% online courses

Start instantly and learn at your own schedule.
Calendar

Flexible deadlines

Reset deadlines in accordance to your schedule.
Beginner Level

Beginner Level

Clock

Approx. 14 hours to complete

Suggested: 6 hours/week...
Comment Dots

Chinese (Simplified)

Subtitles: Chinese (Simplified)...

Syllabus - What you will learn from this course

Week
1
Clock
1 hour to complete

实验一 模式动物与遗传学

包括线虫、果蝇、斑马鱼的雌雄识别(遗传杂交前必备的技能)并了解其生活史(设计实验的同时,倒推实验材料的准备、遗传杂交的操作以及观察后代的各个时间节点必备知识)。...
Reading
6 videos (Total 38 min), 1 quiz
Video6 videos
1.解剖镜的使用方法1m
2线虫的形态观察和雌雄识别2m
3果蝇的雌雄识别1m
4 斑马鱼雌雄形态观察和产卵演示2m
5 斑马鱼卵的形态观察1m
Quiz1 practice exercise
染色体观察26m
Week
2
Clock
1 hour to complete

实验二 染色体观察及果蝇平衡染色体

通过微观(观察不同物种染色体玻片标本)和宏观(观察携带显性标记基因的平衡染色体果蝇[Balancer]表型,包括第一、第二、第三平衡染色体果蝇)水平染色体观察,了解染色体是代与代之间遗传信息传递的载体。通过追踪染色体上的表型,准确无误地区分携带不同基因型的后代,进而定位和追踪基因。涉及最常用的平衡染色体工具果蝇,以及如何利用染色体倒位从而抑制重组的知识点和原理。显微镜与体视解剖镜的使用。...
Reading
4 videos (Total 59 min)
Video4 videos
染色体观察及果蝇平衡染色体(2)26m
1 显微镜观察染色体6m
2 平衡染色体果蝇观察1m
Week
3
Clock
1 hour to complete

实验三 果蝇唾腺染色体制备及观察

提供详细的果蝇唾腺染色体制备演示视频,通过亲自解剖果蝇的唾腺组织,在载玻片上经过一系列的处理制备唾腺染色体,体验简便的唾腺染色体制备方法。实验材料可以选择黑腹果蝇Drosophila melanogaster,也可以选择大果蝇Drosophila virilis。视频讲解和PPT都提供根据端部横纹特征准确无误识别2个物种的每条染色体的清晰图谱。...
Reading
4 videos (Total 62 min), 1 quiz
Video4 videos
果蝇的唾腺染色体制备及观察(2)19m
果蝇的唾腺染色体制备及观察(3)19m
唾腺染色体压片制备演示8m
Quiz1 practice exercise
果蝇唾液腺染色体制备及观察 quiz22m
Week
4
Clock
1 hour to complete

实验四 黑腹果蝇雌雄鉴别与突变体观察

介绍黑腹果蝇在生物学上的分类地位及作为模式动物在遗传学及生物学研究中的地位、介绍识别雌雄及各种突变体的要点和突变体的重要性,观察代表性突变体的同时区别雌雄。...
Reading
2 videos (Total 44 min), 1 quiz
Video2 videos
黑腹果蝇的雌雄鉴别与突变体观察-223m
Quiz1 practice exercise
黑腹果蝇突变体表型鉴别28m

Instructor

张文霞

Associate Professor Wenxia Zhang, PhD
School of Life Sciences

About Peking University

Peking University is determined to make its education openly accessible to students in China and around the world. With over 3000 faculty members, Peking University offers excellence in teaching and learning. Founded in 1898, Peking University (PKU) was the first national comprehensive university in China. For the past 115 years, with its hundreds of thousands of outstanding alumni, Peking University has made prominent contributions in the humanities and sciences to further China's prosperity and progress....

Frequently Asked Questions

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  • When you purchase a Certificate you get access to all course materials, including graded assignments. Upon completing the course, your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.

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